Journal:

Article Title: Expression and Localization of a Novel Rab Small G Protein (Rab38) in the Rat Lung

doi:

Figure Lengend Snippet: a: Immunohistochemistry of native Rab38 protein for rat alveolar tissue. Perfused and excised rat lungs were rapidly frozen in an OCT compound and cut into 5-μm-thick sections. The sections were fixed with 4% paraformaldehyde for 20 minutes and then with acetone for 30 seconds. A polyclonal anti-rat Rab38 antibody (8 μg/ml) was added as the first antibody. For controls, the same concentration of the antibody supplemented with the synthesized polypeptide in 100-fold molar excess was used. Biotin-labeled goat anti-rabbit IgG antibody was added, followed by horseradish peroxidase-conjugated streptavidin. Color development was performed for ∼3 minutes in the presence of diaminobenzidine and H2O2. The slides were counterstained with hematoxylin stain. A and B: Anti-Rab38 antibody. C and D: Control (antibody plus synthetic peptides). Original magnification: ×400 (A and C). ×1,000 (B and D). b: Immunohistochemisty of native Rab38 protein for rat bronchial tissue (original magnification, ×1,000). Experimental procedures are same as described in a. A: Anti-Rab38 antibody. B: Control (antibody plus synthetic peptides).

Article Snippet: For the Lamp-1, fluorescein isothiocyanate-conjugated affinity-purified donkey anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA) at 1:100 dilution followed by Alexa 488-labeled rabbit anti-fluorescein IgG (Molecular Probes) at 1:200 and Cy3.5-conjugated affinity-purified donkey anti-goat IgG antibody at 1:200 dilution (Rockland, Gilbertsville, PA).

Techniques: Immunohistochemistry, Concentration Assay, Synthesized, Labeling, Staining